nuclease
Gene-cutting enzyme
A nuclease is an enzyme that breaks down nucleic acids (DNA or RNA) by cleaving the bonds between nucleotides.
Nucleases are essential tools in molecular biology for cutting genetic material at specific sites: Restriction nucleases allow scientists to splice genes from one organism into another.
Some nucleases cut from the ends of a strand (exonucleases), others cut within it (endonucleases).
Water-induced bond cleavage
- cleavageTendency to split cleanly
- scissionAct of cutting
- cuttingDividing via sharp edge
- nickingSmall cuts or thefts
digestion · degradation · catalysis · site-specific · double-strand break · sticky end · overhang · fragmentation
Hereditary code molecule
- RNAGene expression molecule
- replicationAct of duplicating precisely
- recombinationGenetic material exchange process
- repairRestore to working order
CRISPR · gene editing · genome · chromosome · mutation · double-strand break · proofreading
Enzyme region binding substrates
- substrateUnderlying supporting material
- cofactorEnzyme-activating compound
- magnesiumAlkaline earth metal
- motifRecurring thematic element
fold · dimer · conformation · pocket · cleft · residue · histidine · aspartate
Making identical copies
- DNA fingerprintingTechnique identifying individuals genetically
- Southern blotDNA transfer and detection method
- plasmidSelf-replicating DNA circle
- vectorQuantity with direction
ligation · digest · genome editing · transgenic · molecular biology · recombinant DNA
hydrolysis
Water-induced bond cleavage
nicking · digestion · degradation · catalysis · site-specific · double-strand break · sticky end · overhang · fragmentation · depolymerization
deoxyribonuclease
DNA-degrading enzyme
- Cas9DNA-cutting enzyme
DNA
Hereditary code molecule
- RNAGene expression molecule
- replicationAct of duplicating precisely
- recombinationGenetic material exchange process
repair · CRISPR · gene editing · genome · chromosome · mutation · double-strand break · proofreading
active site
Enzyme region binding substrates
- substrateUnderlying supporting material
- cofactorEnzyme-activating compound
- magnesiumAlkaline earth metal
motif · fold · dimer · conformation · pocket · cleft · residue · histidine · aspartate
cloning
Making identical copies
- DNA fingerprintingTechnique identifying individuals genetically
- Southern blotDNA transfer and detection method
- plasmidSelf-replicating DNA circle
vector · ligation · digest · genome editing · transgenic · molecular biology · recombinant DNA
- nuclear bombFission or fusion weapon
- nuclear warArmed conflict using fission weapons
- nuclear arsenalStockpile of atomic bombs
- nuclear weaponsExplosive devices using fission
- nuclear powerEnergy from splitting atoms
- nuclear explosionViolent atomic energy release
nuclear, nucleate, nucleation, nucleated, nuclearize, nucleating, nuclearizing