Southern blot
Membrane hybridization technique
A Southern blot is a laboratory technique for detecting specific DNA sequences.
DNA fragments are separated by size, transferred to a membrane, and identified using a labeled probe that binds to the target sequence.
Named after biologist Edwin Southern, the technique inspired the names of related methods: Northern blots (for RNA) and Western blots (for proteins).
Mixing distinct genetic lines
- detectionAct of finding something
- nitrocelluloseAcid-treated cellulose compound
- washingCleaning with liquid
- blockingImpeding forward progress
exposure · developing · protocol · workflow · troubleshooting · optimization · standard operating procedure
Heat-softening of metals
- stringencySeverity of requirements
- hybridizeBreed between distinct types
- chemiluminescenceLight from molecular reactions
- fluorescenceLight emitted after absorption
Study of cellular machinery
- 1975A calendar year
- OxfordHistoric English university town
- nomenclatureSystem of specialized terms
- methodologyFormal procedural framework
Technique identifying individuals genetically
- copy numberInstances of a gene
- gene mappingLocating chromosomal trait positions
- paternity testingGenetic confirmation of fatherhood
- forensic analysisScientific examination for legal proceedings
hybridization
Mixing distinct genetic lines
- detectionAct of finding something
- nitrocelluloseAcid-treated cellulose compound
- washingCleaning with liquid
blocking · exposure · developing · protocol · workflow · troubleshooting · optimization · standard operating procedure
annealing
Heat-softening of metals
- stringencySeverity of requirements
- hybridizeBreed between distinct types
- chemiluminescenceLight from molecular reactions
ethidium bromide
Fluorescent nucleic acid stain
molecular biology
Study of cellular machinery
- 1975A calendar year
- OxfordHistoric English university town
- nomenclatureSystem of specialized terms
DNA fingerprinting
Technique identifying individuals genetically
- copy numberInstances of a gene
- gene mappingLocating chromosomal trait positions
- paternity testingGenetic confirmation of fatherhood
- geneticsDNA, allele, genotype, genes, gene
- DNA AnalysisDNA, DNA profiling, DNA testing, DNA sequencing, DNA fingerprinting
- Bioanalysisassay, assays, analyte, mass spectrometry, chromatography
- blotting
- electrophoresiselectrophoresis, PAGE, acrylamide, ladder, wells
- Lab Techniquescentrifugation, electrophoresis, PCR, streak plate, microscopy
Southern blotPCR
DNA amplification technique
DNA sequencing · FISH · microarray · mass spectrometry · DNA fingerprinting