deoxyribonuclease
Nucleic acid scissor
Deoxyribonuclease is an enzyme that cuts DNA molecules by breaking the bonds between nucleotides.
Scientists use deoxyribonucleases—often shortened to DNases—to fragment DNA for analysis or to eliminate unwanted DNA from samples.
The protocol calls for treating the extract with deoxyribonuclease to remove genomic contamination.
Hereditary code molecule
- double helixTwo intertwined spirals
- base pairTwo matched nucleotides bonded
- chromosomeDNA strand carrying genes
- plasmidSelf-replicating DNA circle
chromatin · adenine · thymine · guanine · cytosine · backbone · strand · sequence
Protein that hastens reactions
- catalystAgent enabling change
- active siteEnzyme region binding substrates
- cofactorEnzyme-activating compound
- magnesiumAlkaline earth metal
calcium · substrate · kinetics · hydrolase · protease · specificity · activation · inhibition
Pre-attack information gathering
- apoptosisProgrammed cell death
- DNA extractionIsolating genetic material chemically
- cystic fibrosisGenetic disorder thickening mucus
- sequencingDetermining element order
mapping · mucolytic · therapeutic · recombinant · diagnostic
Water-induced bond cleavage
- nickShallow surface cut
- scissionAct of cutting
- fragmentationBreaking into smaller pieces
- site-specificTied to one location
recognition · substrate · catalyze · specificity · cutting · breakage · restriction · digestion
DNA
Hereditary code molecule
- double helixTwo intertwined spirals
- base pairTwo matched nucleotides bonded
- chromosomeDNA strand carrying genes
plasmid · chromatin · adenine · thymine · guanine · cytosine · backbone · strand · sequence · genome
enzyme
Protein that hastens reactions
- catalystAgent enabling change
- active siteEnzyme region binding substrates
- cofactorEnzyme-activating compound
magnesium · calcium · substrate · kinetics · hydrolase · protease · specificity · activation · inhibition · zymogen
footprinting
Pre-attack information gathering
- apoptosisProgrammed cell death
- DNA extractionIsolating genetic material chemically
- cystic fibrosisGenetic disorder thickening mucus
sequencing · mapping · mucolytic · therapeutic · recombinant · diagnostic
hydrolysis
Water-induced bond cleavage
- nickShallow surface cut
- scissionAct of cutting
- fragmentationBreaking into smaller pieces
site-specific · recognition · substrate · catalyze · specificity · cutting · breakage · restriction · digestion · degradation