Cas9
Bacterial defense enzyme
Cas9 is a protein that acts as molecular scissors, cutting DNA at specific locations.
It's the key component of CRISPR gene-editing technology, guided by RNA to find and snip target sequences in genetic code.
Scientists use Cas9 to delete, replace, or insert genes: The researchers used Cas9 to knock out the disease-causing mutation.
The name comes from "CRISPR-associated protein 9."
Nonstick aerosol brand
- cleavageTendency to split cleanly
- double-strand breakComplete DNA backbone cleavage
- targetingSelecting as objective
- off-targetMissing the intended mark
Engineering novel living systems
- bioengineeringEngineering applied to biology
- molecular biologyStudy of cellular machinery
- genomicsStudy of entire genetic sequences
- cell cultureLaboratory technique for growing tissue
cloning · recombinant DNA · stem cells · biomedicine · pharmaceuticals · bioinformatics · cell lines · plasmid
Medical DNA modification treatment
- diseaseDisorder impairing normal function
- sickle cellInherited blood cell malformation
- cancer immunotherapyUsing immunity to fight malignancy
- muscular dystrophyGenetic muscle-wasting condition
cystic fibrosis · HIV · malaria · agriculture · livestock · diagnostics · screens
Incapacitating final blow
- insertionPlacing something within
- deletionRemoval of content
- mutationPermanent DNA alteration
- repairRestore to working order
correction · modification · transgene · precision · frameshift · disruption · tagging
PAM
Nonstick aerosol brand
- cleavageTendency to split cleanly
- double-strand breakComplete DNA backbone cleavage
- targetingSelecting as objective
synthetic biology
Engineering novel living systems
- bioengineeringEngineering applied to biology
- molecular biologyStudy of cellular machinery
- genomicsStudy of entire genetic sequences
cell culture · cloning · recombinant DNA · stem cells · biomedicine · pharmaceuticals · bioinformatics · cell lines · plasmid · viral vector
gene therapy
Medical DNA modification treatment
- diseaseDisorder impairing normal function
- sickle cellInherited blood cell malformation
- cancer immunotherapyUsing immunity to fight malignancy
muscular dystrophy · cystic fibrosis · HIV · malaria · agriculture · livestock · diagnostics · screens
knockout
Incapacitating final blow
repair · correction · modification · transgene · precision · frameshift · disruption · tagging
thermostable
Unaffected by high temperatures