spectrophotometry
Wavelength-based chemical analysis
Spectrophotometry is the measurement of how much light a substance absorbs or transmits at different wavelengths.
The technique identifies chemicals and determines their concentrations—widely used in chemistry, biochemistry, and clinical laboratories: Spectrophotometry confirmed elevated bilirubin levels in the blood sample.
A spectrophotometer is the instrument that performs these measurements.
Light uptake measurement
- transmittanceFraction not absorbed
- concentrationSustained single-minded attention
- calibrationAdjusting for measurement accuracy
- blankWithout marks or content
linearity · quantitative · assay · molarity · sample · dilution · baseline · analyte
Wavelengths shorter than violet
- visibleAble to be seen
- infraredHeat-bearing electromagnetic waves
- nanometerMetric unit, billion-fold smaller
- monochromaticOf one hue
bandwidth · tungsten · LED · filter · near-infrared
Device that senses
- photodiodeLight-to-current semiconductor
- opticsScience of illumination
- lensLight-focusing curved glass
- mirrorReflective viewing surface
grating · diffraction · resolution · signal-to-noise · sensitivity · spectrophotometer · benchtop · portable
City in northern England
- pharmaceuticalManufactured medicinal compound
- turbidityCloudiness from suspended particles
- hemoglobinErythrocyte's oxygen-binding protein
- chlorophyllLight-harvesting plant pigment
absorbance
Light uptake measurement
- transmittanceFraction not absorbed
- concentrationSustained single-minded attention
- calibrationAdjusting for measurement accuracy
blank · linearity · quantitative · assay · molarity · sample · dilution · baseline · analyte · titration
ultraviolet
Wavelengths shorter than violet
- visibleAble to be seen
- infraredHeat-bearing electromagnetic waves
- nanometerMetric unit, billion-fold smaller
monochromatic · bandwidth · tungsten · LED · filter · near-infrared
detector
Device that senses
- photodiodeLight-to-current semiconductor
- opticsScience of illumination
- lensLight-focusing curved glass
mirror · grating · diffraction · resolution · signal-to-noise · sensitivity · spectrophotometer · benchtop · portable · scanning
Bradford
City in northern England
- pharmaceuticalManufactured medicinal compound
- turbidityCloudiness from suspended particles
- hemoglobinErythrocyte's oxygen-binding protein
- Chemical Analysischemical analysis, titration, chromatography, mass spectrometry, gravimetry
- spectroscopyspectrometer, spectrograph, spectrogram, spectrophotometer, spectrometry
- Bioanalysisassay, assays, analyte, mass spectrometry, chromatography
- Lab Analysisspectrometer, colorimeter, absorbance, analyte, reagent
- Lab MethodsPCR, mass spectrometry, chromatography, microscopy, electrophysiology
- Lab Techniquescentrifugation, electrophoresis, PCR, streak plate, microscopy
spectrophotometrygravimetry
Measuring gravitational field strength
mass spectrometry · calorimetry · manometry · chromatography · electrophoresis
photo, spectrum, spectral, photometry, spectroscopy, photometer, spectroscope, spectra, photosensitive