microinjection
Needle enters cell
Microinjection is a laboratory technique that uses an extremely fine needle to deliver substances directly into individual cells.
Scientists employ it to introduce DNA, RNA, proteins, or other molecules for research purposes, including genetic modification and studying cellular processes.
The method is also used in assisted reproduction, where a single sperm cell is injected into an egg.
The researchers used microinjection to insert the gene into embryonic stem cells.
Gel surrounding the nucleus
- transfectionNon-viral gene introduction
- oocyteUndeveloped female gamete
- embryoOrganism before birth
- somaticConcerning the body
cell membrane · nucleus · organelle · blastocyst · zygote · protoplast · single-cell
Gamete fusion creating zygote
- assisted reproductionMedical conception methods
- infertilityInability to reproduce
- spermMale reproductive cell
- oocyteUndeveloped female gamete
embryo transfer · IVF · pronuclear · blastomere · cryopreservation
Smallest blood vessel
- needlePointed implement for sewing
- micromanipulatorDevice for microscopic positioning
- manipulationUnderhanded control of others
- microscopyTechnique using powerful lenses
nanoliter · glass · steady · dexterity · calibrated · targeted · precision · control
Gene-editing molecular machinery
- transfectionNon-viral gene introduction
- transgenicHaving inserted foreign genes
- gene editingTargeted DNA alteration
- plasmidSelf-replicating DNA circle
mRNA · knockout · zebrafish · Drosophila · phenotype · genotype · expression · laboratory
Within skin tissue
- collagenaseEnzyme degrading fibrous protein
- dermal fillerSubstance injected beneath skin
- BotoxCosmetic paralytic injection
- subcutaneousBelow the skin
cytoplasm
Gel surrounding the nucleus
- transfectionNon-viral gene introduction
- oocyteUndeveloped female gamete
- embryoOrganism before birth
somatic · cell membrane · nucleus · organelle · blastocyst · zygote · protoplast · single-cell
fertilization
Gamete fusion creating zygote
- assisted reproductionMedical conception methods
- infertilityInability to reproduce
- spermMale reproductive cell
oocyte · embryo transfer · IVF · pronuclear · blastomere · cryopreservation
capillary
Smallest blood vessel
- needlePointed implement for sewing
- micromanipulatorDevice for microscopic positioning
- manipulationUnderhanded control of others
microscopy · nanoliter · glass · steady · dexterity · calibrated · targeted · precision · control · delicate
CRISPR
Gene-editing molecular machinery
- transfectionNon-viral gene introduction
- transgenicHaving inserted foreign genes
- gene editingTargeted DNA alteration
plasmid · mRNA · knockout · zebrafish · Drosophila · phenotype · genotype · expression · laboratory · biomedical
intradermal
Within skin tissue
- collagenaseEnzyme degrading fibrous protein
- dermal fillerSubstance injected beneath skin
- BotoxCosmetic paralytic injection