transfection
Inserting foreign DNA
Transfection is the process of introducing foreign genetic material into cells, typically DNA or RNA into animal cells grown in culture.
Scientists use transfection to study gene function, produce proteins, or develop gene therapies: We transfected the cells with a plasmid encoding green fluorescent protein.
The term distinguishes this technique from transformation (bacteria) and transduction (viruses).
Self-replicating DNA circle
- cell linePropagated cells from ancestor
- transientTemporary, soon departing
- calcium phosphateMineral compound in bones
- viral vectorVirus repurposed for therapy
knockdown · overexpression · CRISPR · microinjection · construct
Tumor-inducing genetic element
- carcinogenCancer-promoting agent
- metastasisCancer spreading beyond origin
- apoptosisProgrammed cell death
- angiogenesisFormation of new vessels
mutation · tumor · melanoma · osteosarcoma · papillomavirus · retrovirus · herpesvirus · telomerase
plasmid
Self-replicating DNA circle
- cell linePropagated cells from ancestor
- transientTemporary, soon departing
- calcium phosphateMineral compound in bones
viral vector · knockdown · overexpression · CRISPR · microinjection · construct
oncogene
Tumor-inducing genetic element
- carcinogenCancer-promoting agent
- metastasisCancer spreading beyond origin
- apoptosisProgrammed cell death
angiogenesis · mutation · tumor · melanoma · osteosarcoma · papillomavirus · retrovirus · herpesvirus · telomerase · gene
infect, infection, disinfect, infectious, disinfectant, disinfection, infective, reinfection, gene transfer