lysate
Burst cell contents
A lysate is the mixture of cellular contents released when cells are broken open—lysed—through chemical, enzymatic, or mechanical means.
Cell lysates are fundamental to molecular biology, allowing researchers to extract and study proteins, DNA, and other cellular components: We prepared a bacterial lysate for protein analysis.
Making things the same
- sonicationSound-based particle agitation
- centrifugationSpinning to separate densities
- supernatantLiquid above precipitate
- pelletSmall rounded mass
buffer · detergent · solubilization · fractionation · clarification · filtration · freeze-thaw · French press
Amino acid polymers
- nucleic acidsBiopolymers carrying genetic code
- lipidsWater-insoluble biomolecules
- cytoplasmGel surrounding the nucleus
- organellesSpecialized cell subunits
ribosomes · mitochondria · enzymes · metabolites · DNA · RNA · chromatin · cytoskeleton
Measures ionic weight ratios
- electrophoresisField-driven particle separation
- spectrophotometryMeasuring light wavelength absorption
- chromatographyTechnique separating mixtures by differential migration
- pull-downMenu that descends
Tests identifying conditions
- drug screeningTest for prohibited substances
- quality controlMaintaining production standards
- batch processingRunning queued jobs automatically
homogenization
Making things the same
- sonicationSound-based particle agitation
- centrifugationSpinning to separate densities
- supernatantLiquid above precipitate
pellet · buffer · detergent · solubilization · fractionation · clarification · filtration · freeze-thaw · French press
proteins
Amino acid polymers
- nucleic acidsBiopolymers carrying genetic code
- lipidsWater-insoluble biomolecules
- cytoplasmGel surrounding the nucleus
organelles · ribosomes · mitochondria · enzymes · metabolites · DNA · RNA · chromatin · cytoskeleton · antibodies
mass spectrometry
Measures ionic weight ratios
- electrophoresisField-driven particle separation
- spectrophotometryMeasuring light wavelength absorption
- chromatographyTechnique separating mixtures by differential migration
diagnostic testing
Tests identifying conditions
- drug screeningTest for prohibited substances
- quality controlMaintaining production standards
- batch processingRunning queued jobs automatically
analysis, paralysis, dialysis, catalysis, lysis, hydrolysis, lysosome, lyse, electrolysis