Beer-Lambert law
Light absorption relationship
The Beer-Lambert law describes how light intensity decreases as it passes through a material that absorbs it.
The absorbance is proportional to both the concentration of the absorbing substance and the path length the light travels.
It's fundamental in spectroscopy for determining concentrations of solutions: Using the Beer-Lambert law, we calculated the protein concentration from the absorbance reading.
Light uptake measurement
- attenuationGradual intensity reduction
- chromophoreMolecule's tint-causing portion
- transmittanceFraction not absorbed
- light absorptionMatter capturing radiant energy
absorptivity · electromagnetic radiation · photon · ultraviolet · infrared · visible spectrum · energy levels · deviation
Light-measuring analytical instrument
- analyteSubstance being measured
- wavelengthSpatial repetition interval
- photodiodeLight-to-current semiconductor
- detectorDevice that senses
Of one hue
- collimatedMade into parallel beams
- refractionWave bending between media
- scatteringDistribution in many directions
- photometryMeasuring light intensity
irradiance · intensity · flux · interference · medium · beam · transmission
Study of matter's composition
- environmental monitoringObserving natural systems systematically
- water qualityCondition of H2O supply
- blood analysisMedical fluid examination
- atmospheric scienceStudy of Earth's atmosphere
absorbance
Light uptake measurement
- attenuationGradual intensity reduction
- chromophoreMolecule's tint-causing portion
- transmittanceFraction not absorbed
light absorption · absorptivity · electromagnetic radiation · photon · ultraviolet · infrared · visible spectrum · energy levels · deviation · saturation
spectrophotometer
Light-measuring analytical instrument
- analyteSubstance being measured
- wavelengthSpatial repetition interval
- photodiodeLight-to-current semiconductor
monochromatic
Of one hue
- collimatedMade into parallel beams
- refractionWave bending between media
- scatteringDistribution in many directions
photometry · irradiance · intensity · flux · interference · medium · beam · transmission
analytical chemistry
Study of matter's composition
- environmental monitoringObserving natural systems systematically
- water qualityCondition of H2O supply
- blood analysisMedical fluid examination
- Chemistry LawsAvogadro's law, Boyle's law, gas laws, ideal gas law, second law of thermodynamics
- Lab Analysisspectrometer, spectrophotometry, spectroscopy, colorimeter, photometer
- spectroscopyspectrum, spectrometer, spectroscope, spectrograph, spectrogram
- absorption spectraspectral lines, Fraunhofer lines, dark lines, Balmer series, absorbance
Beer-Lambert lawMie scattering
Light scattered by larger particles
light scattering · fluorescence · phosphorescence · light emission · specular reflection