spectrophotometers
Wavelength measurement devices
Spectrophotometers are instruments that measure how much light a substance absorbs or transmits at different wavelengths.
They're fundamental tools in chemistry, biology, and materials science for identifying compounds, measuring concentrations, and analyzing color: The spectrophotometer showed elevated protein levels in the sample.
The device passes light through a sample and compares intensity before and after.
Light uptake measurement
- transmittanceFraction not absorbed
- wavelengthSpatial repetition interval
- concentrationSustained single-minded attention
- Beer-Lambert lawRelates absorbance to concentration
molarity · quantification · blank · reference · dynamic range
Light-to-current semiconductor
- detectorDevice that senses
- light sourceWhat gives off illumination
- beam splitterDivides incident light
- filterPorous separation device
prism · slit · mirror · lens · fiber optic
Degree of correctness
- precisionAccuracy of measurement
- reproducibilityAbility to repeat findings
- sensitivityResponsiveness to stimuli
- resolutionFormal decision or determination
specificity · linearity · noise · drift · stability · calibration · validation · quality control
Wavelengths shorter than violet
- visibleAble to be seen
- infraredHeat-bearing electromagnetic waves
- near-infraredRadiation just past visible
- nanometersBillionths of meters
bandwidth · monochromatic · polychromatic · peak · trough · scanning
Condition of H2O supply
- colorimetryMeasuring perceived hues precisely
- turbidityCloudiness from suspended particles
- environmental monitoringObserving natural systems systematically
absorbance
Light uptake measurement
- transmittanceFraction not absorbed
- wavelengthSpatial repetition interval
- concentrationSustained single-minded attention
Beer-Lambert law · molarity · quantification · blank · reference · dynamic range
photodiode
Light-to-current semiconductor
- detectorDevice that senses
- light sourceWhat gives off illumination
- beam splitterDivides incident light
accuracy
Degree of correctness
- precisionAccuracy of measurement
- reproducibilityAbility to repeat findings
- sensitivityResponsiveness to stimuli
resolution · specificity · linearity · noise · drift · stability · calibration · validation · quality control · standards
ultraviolet
Wavelengths shorter than violet
- visibleAble to be seen
- infraredHeat-bearing electromagnetic waves
- near-infraredRadiation just past visible
nanometers · bandwidth · monochromatic · polychromatic · peak · trough · scanning
water quality
Condition of H2O supply
- colorimetryMeasuring perceived hues precisely
- turbidityCloudiness from suspended particles
- environmental monitoringObserving natural systems systematically