primary antibody
Initial target recognizer
A primary antibody is an antibody that binds directly to a target molecule—typically a protein or antigen—in laboratory techniques like immunohistochemistry or Western blotting.
It provides the specific recognition, while a secondary antibody binds to the primary and carries the detection signal: The primary antibody targets the protein of interest; the secondary antibody makes it visible.
- antigenImmune response inducer
- epitopeAntibody binding region
- fluorescentEmitting light when energized
- detectionAct of finding something
- bindingLegally or morally required
specificity · monoclonal · host species · rabbit · mouse · incubation · blocking · wash · conjugate · affinity · catalog number · lot number · data sheet · stock solution · dilution · overnight · cold room · room temperature
- Immunostainingantigen, DAB, chromogen, fluorescein, biotin
- antibodiesheavy chain, nanobodies, valency, cross-reactivity, CDR
- immunolabeling
- lab reagentsreagent, buffer, buffers, formalin, pyridine
- immunoassaysantigen test, lateral flow test, EIA, VDRL test, Wassermann test