secondary antibody
Binds primary antibody
A secondary antibody is an antibody that binds to other antibodies rather than directly to an antigen.
In laboratory techniques like Western blots and immunohistochemistry, secondary antibodies detect primary antibodies that have already bound to a target.
They're typically conjugated to enzymes or fluorescent markers for visualization: The secondary antibody was labeled with horseradish peroxidase.
- conjugateModify verbs grammatically
- detectionAct of finding something
- bindingLegally or morally required
- goatHorned, bearded livestock
- rabbitLong-eared lagomorph
mouse · signal boost · epitope · fluorescent · labeled · probe · visualization · staining · background noise · blocking · wash · incubation · target · specificity · host species · cross-reactivity · chemiluminescence
- Immunostainingantigen, DAB, chromogen, fluorescein, biotin
- antibodiesheavy chain, nanobodies, valency, cross-reactivity, CDR
- immunolabeling
- lab reagentsreagent, buffer, buffers, formalin, pyridine
- fluorescence microscopyfluorescent dye, fluorescein, CFP, confocal, spinning disk
- immunoassaysantigen test, lateral flow test, EIA, VDRL test, Wassermann test
secondary antibodyprimary antibody
Antigen-binding immunoglobulin in assays
secondary, second, secondly, secondarily, antibody, antibodies, bodily